Characterization of Cellulose Fibers According to Cellulase-Active Microbial Pretreatment

Kyojung Hwang; Jisoo Park; Sang-Jin Chun; Yujin Oh; Jieun Lee; Tai-Ju Lee; Jaegyoung Gwon

doi:10.7584/JKTAPPI.2023.4.55.2.19

All Issue

2023 Vol.55, Issue 2 Preview Page Next Page

Research Article

Characterization of Cellulose Fibers According to Cellulase-Active Microbial Pretreatment Cellulase 활성 미생물 전처리에 따른 셀룰로오스 섬유의 특성 변화

30 April 2023. pp. 19-29

PDF XML

Abstract

This study evaluated the effects of microbial (Bacillus subtilis, Bacillus licheniformis) pretreatment and mechanical (ball mill) treatment on the nanofibrillation of cellulose. The optimal growth conditions were identified a B. subtilis (pH 6-8, 25℃ and 100 rpm) and B. licheniformis (pH 7, 35℃ and 150 rpm), respectively. As a result of evaluating microbial reducing sugar production from carboxymethyl cellulose, B. subtilis case showed similar trends at most experimental conditions (pH, temperature and rpm) except for pH 5. The maximum amount of reducing sugar by B. licheniformis was achieved at the culture conditions with pH 7, 35℃ and 150 rpm. There was no change in the crystalline structure of cellulose by ball mill and microbial treatment, but, XRD peaks intensity decreased. Cellulose nanofibers were observed after 6 hours ball mill treatment of alpha cellulose with/without the microbial pretreatment. When applying microbial pretreatment, the cellulose nanofibers produced by the ball mill had more longer in length than those without the pretreatment.

Keywords

Bacillus subtilis

Bacillus licheniformis

growth condition

reducing sugar

cellulose nanofiber

References

Aziz, T., Farid, A., Haq, F., Kiran, M., Ullah, A., Zhang, K., Li, C., Ghazanfar S., Sun, H., Ullah R., Ali, A., Muzammal, M., Shah, M., Akhtar, N., Selim, S., Hagagy, N., Samy M., and Al Jaouni S. K., A review on the modification cellulose and its applications, Polymers 14(15):3206-3239 (2022). 10.3390/polym1415320635956720PMC9371096

Yoon, H. J., Gil, B. M., Lee, J. H., Park, J. E., Lim, J., Jo, M. J., Jung, K., and Wie J. J., Thermal and mechanical properties of polypylene/cellulose nanofiber composites, Polymer (Korea) 44(3):255-263 (2020). 10.7317/pk.2020.44.3.255

Kim, Y. S., Yu, S. M., and Park C. S., Screening of cellulolytic microorganism and characterization of cellulase from Bacillus subtilis PFB-1, Journal of Chitin and Chitosan 24(3): 179-185 (2019). 10.17642/jcc.24.3.5

Alokika, Kumar, V., and Singh B., Biochemical characteristics of a novel ethanol-tolerant xylanase from Bacillus subtilis subsp. subtilis JJBS250 and its applicability in saccharification of rice straw, Biomass Conversion and Biorefinery 13:1937-1949 (2023). 10.1007/s13399-020-01257-0

Arsov, A., Petrov, K., and Petrova, P., Enhanced activity by genetic complementarity: Heterologous secretion of clostridial cellulases by Bacillus licheniformis and Bacillus velezensis, Molecules 26(18):5625-5639 (2021). 10.3390/molecules2618562534577096PMC8468253

Ou, M. S., Mohammed, N., Ingram, L. O., and Shanmugam K. T., Thermophilic Bacillus coagulans requires less cellulases for simultaneous saccharification and fermentation of cellulose to products than mesophilic microbial biocatalysts, Applied Biochemistry and Biotechnology 155:76-82 (2009). 10.1007/s12010-008-8509-419156365

Ye, M., Sun, L., Yang, R., Wang, Z., and Qi, K. Z., The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed, Royal Society Open Science 4:171012- 171023 (2017). 10.1098/rsos.17101229134097PMC5666280

Tabssum, F., Irfan, M., Shakir, H. A., and Qazi, J. I., RSM based optimization of nutritional conditions for cellulase mediated Saccharification by Bacillus cereus, Journal of Biological Engineering 12(1):1-10 (2018). 10.1186/s13036-018-0097-429755582PMC5934882

Li, Y., Li, G., Zhao, X., Shao, Y., Wu, M., and Ma, T., Regulation of hyaluronic acid molecular weight and titer by temperature in engineered Bacillus subtilis, 3 Biotech 9(6): 225-233 (2019). 10.1007/s13205-019-1749-x31139540PMC6529495

Xiong, Y., Wang, Y., Yu, Y., Li, Q., Wang, H., Chen, R., and He, N., Production and characterization of a novel bioflocculant from Bacillus licheniformis, Applied and Environmental Microbiology 76(9):2778-2782 (2010). 10.1128/AEM.02558-0920208025PMC2863459

Goyal, V., Mittal, A., Bhuwal, A. K., Singh, G., Yadav, A., and Aggarwal, N. K., Parametric optimization of cultural conditions for carboymethyl cellulase production using pretreated rice straw by Bacillus sp. 313SI under stationary and shaking conditions, Biotechnology Research International 2014:1-7 (2014). 10.1155/2014/65183924868469PMC4020544

Doan, C. T., Tran, T. N., Nguyen, T. T., Tran, T. H., Nguyen, V. B., Tran, T. D., Nguyen, A. D., and Wang, S. L., Production of sucrolytic enzyme by Bacillus licheniformis by the bioconversion of pomelo albedo as a carbon source, Polymers 13(12):1959-1976 (2021). 10.3390/polym1312195934199171PMC8231626

Fatani, S., Saito, Y., Alarawi, M., Gojobori, T., and Mineta K., Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the red sea, BMC Microbiology 21:254-265 (2021). 10.1186/s12866-021-02316-w34548024PMC8456639

Andlar, M., Reic, T., Mardetko, N., Kracher, D., Ludwig, R., and Santek, B., Lignocellulose degradation: An overview of fungi and fungal enzymes involved in lignocellulose degradation, Engineering in Life Sciences 18(11): 768-778 (2018). 10.1002/elsc.20180003932624871PMC6999254

Costa, M. N., Beigas, B., Jacob, J. M., Santos, D. S., Gomes, J., Baptista, P. V., Martins, R., Inacio, J., and Fortunato, E., A low cost, safe, disposable, rapid and self-sustainable paper-based platform for diagnostic testing: lab-on-paper, Nanotechnology 25(9):94006- 94017 (2014). 10.1088/0957-4484/25/9/09400624521980

Beyene, D., Chae, M., Dai, J., Danumah, C., Tosto, F., Demesa, A. G., and Bressler, D. C., Characterization of cellulase-treated fibers and resulting cellulose nonocrystals generated through acid hyrolysis, Materials 11(8):1272-1287 (2018). 10.3390/ma1108127230042345PMC6117684

Smirnov, M. V., and Loginova, S. V., 3D model of short-range order of one-hour milled cellulose, Journal of Physics: Conference Series 1038:12059-12062 (2018). 10.1088/1742-6596/1038/1/012059

Rao, X., Kuga, S., Wu, M., and Huang, Y., Influence of solvent polarity on surface-fluorination of cellulose nanofiber by ball milling, Cellulose 22:2341-2348 (2015). 10.1007/s10570-015-0659-2

Dias, I. K. R., Siqueira, G. G., and Arantes, V., Xylanase increases the selectivity of the enzymatic hydrolysis with endoglucanase to produce cellulose nanocrystals with improved properties, International Journal of Biological Macromolecules 220:589-600 (2022). 10.1016/j.ijbiomac.2022.08.04735963352

Information

Publisher :Korea Technical Association of The Pulp and Paper Industry
Publisher(Ko) :한국펄프종이공학회
Journal Title :Journal of Korea TAPPI
Journal Title(Ko) :펄프종이기술
Volume : 55
No :2
Pages :19-29
Received Date : 2023-03-24
Revised Date : 2023-04-10
Accepted Date : 2023-04-12
DOI :https://doi.org/10.7584/JKTAPPI.2023.4.55.2.19

Journal of Korea TAPPIISSN:0253-3200(Print)한국펄프종이공학회

All Issue