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2024 Vol.56, Issue 5 Preview Page

Research Article

Production, Purification and Decolorization of Microbial Levan Using Fallen-Leaves Extract Medium

낙엽추출물 배지를 이용한 미생물 레반의 생산, 정제 및 탈색

Sangjun Lee1
,
Hyunjun Ko2†
이 상준1
,
고 현준2†
1Dept. of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, 1 Gangwondaehak-gil, Chuncheon 24341, Republic of Korea
2Dept. of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, 1 Gangwondaehak-gil, Chuncheon 24341, Republic of Korea
1강원대학교 산림환경과학대학 산림바이오소재공학과, 대학원생
2강원대학교 산림환경과학대학 산림바이오소재공학과, 조교수
†Corresponding author: E-Mail: kohney@kangwon.ac.kr (Address: Dept. of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, 1 Gangwondaehak-gil, Chuncheon 24341, Republic of Korea)
30 October 2024. pp. 58-65
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Abstract

Fallen leaves are often viewed as nuisance unused forest biomass, contributing to wildfire risks and sewer blockages. However, they also present a valuable resource for producing high-value-added products. In a previous study, we demonstrated the economic feasibility of cultivating Pseudomonas species microorganisms using a hardwood fallen leaf extract (HLE)-based medium. This study focuses on further utilization of the HLE medium to produce levan, a functional natural polymer, by addition of sucrose as a carbon source and cultivation of Pseudomonas chlororaphis. Over a 72-hour cultivation, the cell growth-dependent levan production was monitored, resulting in a yield of 7.1 g/L from 19.6 g/L of sucrose (36.7% conversion yield). The crude levan was purified through a straightforward filtration process. However, the purified levan exhibited a dark-brown color due to the inherent pigmentation of the HLE, which could limit its industrial applications. To address this, we conducted decolorization experiments using eight different materials, with activated charcoal designed for water treatment showing the highest efficacy. Under the optimal decolorization conditions, the produced levan achieved a clear, colorless appearance. Taken together, this study confirms the industrial feasibility of using fallen leaf-based media for the production of high-value bioproducts like levan.

Keywords
Fallen-leaf extract
microbial media
Pseudomonas chlororaphis
levan
decolorization
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Information
  • Publisher :Korea Technical Association of The Pulp and Paper Industry
  • Publisher(Ko) :한국펄프종이공학회
  • Journal Title :Journal of Korea TAPPI
  • Journal Title(Ko) :펄프종이기술
  • Volume : 56
  • No :5
  • Pages :58-65
  • Received Date : 2024-09-30
  • Revised Date : 2024-10-11
  • Accepted Date : 2024-10-11
  • DOI :https://doi.org/10.7584/JKTAPPI.2024.10.56.5.58
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