Enzyme Activity and Beating Properties for Preparation of MicroFibrillated Cellulose(MFC)

Kang-Jae Kim; Jin-Dong Jung; Soo-Eune Jung; Eun-Byeoul Ahn; Tae-Jin Eom

doi:10.7584/ktappi.2015.47.1.059

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Enzyme Activity and Beating Properties for Preparation of MicroFibrillated Cellulose(MFC) MicroFibrillated Cellulose(MFC) 제조를 위한 전처리 효소의 활성 및 고해 특성

28 February 2015. pp. 59-65

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Abstract

In this study, we evaluated optimum condition of enzyme with pH and temperature for preparation of microfibillated cellulose(MFC). Well-known endo-glucanase, three enzymes were used and CMC was used for substrate. Enzyme activity was evaluated using DNS method and absorbance with UV/VIS spectrophotometer. The enzyme shown the greatest activity was reacted with pulps at optimum condition for 1 hour and treated pulps beated until 100 mL CSF. Enzyme B and Enzyme L was the higher enzyme activity below 0.1% concentration and Enzyme N was the lowest enzyme activity. At various pH and temperature conditions, enzyme activity of Enzyme B was higher than the others at the same concentration. Especially enzyme activity at 50℃ of Enzyme B was almost not changed over pH 6.0. Optimum condition of three enzyme was pH 6 or pH 7 and 50℃or 60℃. Also beating efficiency of enzyme treated pulps with Enzyme B is 55.6%.

Keywords

micro fibrillated cellulose(MFC)

endo-glucanase

enzyme activity

optimum condition

beating efficiency

References

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Information

Publisher :Korea Technical Association of The Pulp and Paper Industry
Publisher(Ko) :한국펄프종이공학회
Journal Title :Journal of Korea TAPPI
Journal Title(Ko) :펄프종이기술
Volume : 47
No :1
Pages :59-65
Received Date : 2015-01-14
Revised Date : 2015-02-13
Accepted Date : 2015-02-23
DOI :https://doi.org/10.7584/ktappi.2015.47.1.059

Journal of Korea TAPPI ISSN:0253-3200(Print) 펄프종이기술

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Literature Cited