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2025 Vol.57, Issue 6 Preview Page

Original Paper

Optimization of Bacterial Nanocellulose Production from Sweet Potato Waste via Response Surface Methodology
Lili Melani1†
,
Grace Brigitta Gunawan2
,
Reso Aji Saputra Wicaksono2
,
Gilang Mahendra Pratama2
1School of Life Science and Technology, Institut Teknologi Bandung, Professor
2School of Life Science and Technology, Institut Teknologi Bandung, Students
†Corresponding Author: E-mail: lilimelani@itb.ac.id (Address: School of Life Science and Technology, Institut Teknologi Bandung, West Java, 40132, Indonesia)
30 December 2025. pp. 41-55
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Abstract

Cilembu sweet potato is a high-yielding Indonesian variety that generates considerable agro-industrial waste from unconsumed biomass. Owing to its high carbohydrate content, this waste hydrolysate was evaluated as a carbon source for the cultivation of Gluconacetobacter xylinus to produce bacterial nanocellulose (BNC). Production parameters were optimized using a Box–Behnken experimental design to assess the effects of initial pH, bacterial inoculum volume, and D-glucose concentration on BNC yield. Statistical analysis indicated that both pH and inoculum concentration significantly influenced yield, whereas D-glucose concentration had no statistically significant effect (p = 0.059). Optimal conditions were identified at pH 5.7 and 10% inoculum, yielding 3.97 g/L of BNC. The synthesized BNC consisted of interconnected nanofibers of 10–100 nm in diameter, with a crystallinity index of up to 77%. Collectively, these results demonstrate that Cilembu sweet potato waste hydrolysate represents an efficient and sustainable carbon substrate for BNC biosynthesis, eliminating the need for additional glucose supplementation.

Keywords
Cilembu sweet potato
Gluconacetobacter xylinus
bacterial nanocellulose
optimization
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Information
  • Publisher :Korea Technical Association of The Pulp and Paper Industry
  • Publisher(Ko) :한국펄프종이공학회
  • Journal Title :Journal of Korea TAPPI
  • Journal Title(Ko) :펄프종이기술
  • Volume : 57
  • No :6
  • Pages :41-55
  • Received Date : 2025-08-18
  • Revised Date : 2025-10-20
  • Accepted Date : 2025-10-20
  • DOI :https://doi.org/10.7584/JKTAPPI.2025.12.57.6.41
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