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2017 Vol.49, Issue 4 Preview Page
Evaluation of Data Reliability in the Micro-scale Compositional Neutral Sugar Analysis of Lignocellulosic Biomass

미량 시료를 이용한 목질 바이오매스의 중성 당 조성 분석 데이터의 신뢰성 평가

Yeseul Doo
,
Myung-Joon Jeong1
,
Ju-Hyun Yu2
,
Kyu-Young Kang†
두예슬
,
정명준 1
,
유주현 2
,
강규영 †
Department of Biological and Environmental Science, College of Life Science and Biotechnology, Dongguk University-Seoul, Goyang, 10326
1Department of Wood Science and Technology, College of Agricultural Life Science, Chonbuk National University, Jeonju, 54896
1Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology, Daejeon, 34114
동국대학교 바이오시스템대학 바이오환경과학과
1전북대학교 농업생명과학대학 목재응용과학과
1한국화학연구원 바이오화학연구센터
†Corresponding author: E-Mail: kykang@dongguk.edu
30 August 2017. pp. 82-88
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Abstract

In this study, a micro-scale analytical method for compositional neutral sugars in lignocellulosic biomass was evaluated to obtain the high reproducibility of analytical data when compared to general chemical analysis, with the purpose of setting up a chemical analysis using micro-destructive method that possesses reproducibility as high as general analysis methods. The limits of detection (LOD) and the limits of quantification (LOQ) were 4.3-5.9 mg/L and 13.1-17.9 mg/L, respectively. Loss factor of 2nd acid hydrolysis of sample was higher than that of 1st acid hydrolysis. The error range of the relative neutral sugar composition between normal destructive and micro-destructive method was about ±0.2%. However, the error of absolute glucose content was about ±0.7% caused by weighing errors. The accuracy and precision of an electronic scale was showed as the most important factor when using micro-scale samples. The results showed that the micro- scale analytical method can be suitably applied to the neutral sugar analysis of lignocellulosic biomass.

Keywords
Lignocellulosic biomass
micro-destructive analysis
acid hydrolysis
neutral sugars
data reliability
HPLC
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Information
  • Publisher :Korea Technical Association of The Pulp and Paper Industry
  • Publisher(Ko) :한국펄프종이공학회
  • Journal Title :Journal of Korea TAPPI
  • Journal Title(Ko) :펄프종이기술
  • Volume : 49
  • No :4
  • Pages :82-88
  • Received Date : 2017-08-05
  • Revised Date : 2017-08-21
  • Accepted Date : 2017-08-22
  • DOI :https://doi.org/10.7584/JKTAPPI.2017.08.49.4.82
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